Shared architecture of bacteriophage SPO1 and herpesvirus capsids

For initial 3D analysis, negative-stain images of partially purified phage contrasted with 1% ammonium molybdate were taken on a Zeiss 901 operating at 80 kV and magnification of 30,000x. Micrographs were digitized at a rate of 14 μm, corresponding to 4.7 Å at the sample. Selected images were analyzed by self-common lines to generate a coarse model from a single particle that was used to begin orientation searches for the cryoEM data. Negative-stain images contrasted with uranyl acetate were taken with an FEI CM12 microscope operating at 120 kV and magnification of 28,000x.